In a laboratory and research centres, there will be need of various equipment, science products and chemicals to do varied test to diagnosis the problem. Benedict reagent is one of them. This is widely used in maximum laboratory to check the presence of glucose in urine. Are you running and you have regular need of Benedict reagent in your lab then there are many companies those provide the facility of buying lab related all kinds of products online throughout the world. With the help of online suppliers it is easy to order and buy this product anytime.
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What is Benedict’s test?
Benedict’s test is a process of testing for simple carbohydrates / impedance sugars/ disaccharides. It is also known as Benedict’s regent test. This test is used to test for the impendence of glucose in urine, but it is not used for the diagnosis of diabetes. Usually it identifies reducing sugar. In a college day, many of us have read about reducing Sugar. Today I am going to tell you how it can work, so a reducing sugar is any sugar that is competent of acting as a reducing agent because it has a free aldehyde team or a free ketone team. Decreasing sugar redress through the amino acids in the Maillard impedance, the range of impedance that happen until cooking food at high atmosphere and that is necessary in characterizing the tasteful of food.
Theory of Benedict’s test
Its theory is that when decrease sugars are burnet endwise, the resolution changes its colour to orange red or brick red. This colour is suitable for the impedance of simple carbohydrate. The copper (II) ions in Benedict’s solution restrained to copper (I) ions, that reasons pigment change.
Benedict’s reagent solution
This is a similar volume combination of aqueous solution of copper sulphate (cuSO4) and sodium citrate. Sodium is the complexing agent and Benedict’s solution is a deep blue alkaline solution. It is used to test for the impedance of thee aldehyde functional team CHO.
These are few types of carbohydrates like amylum and starch consist of large digit of glucose monomer units mixed by glycosidic bonds. The two carbohydrates Starch and amylum don’t react unhealthy with Benedict’s reagent, due to comparatively small digit of decreasing of sugars units.
Sodium carbohydrate of Benedict’s reagent facilition the alkaline terms which are expected for the redox response. Some of other compound sodium citrates complexes from the copper (II) ions to ignore digression into copper (I) ions until deposit. The Benedict’s test continues the concentration of reducing sugar growth. By following these terms, a large amount of brick red colour quick will be worked at the end of test tube. Anytime you will search small amounts of copper oxide at all with brick- red precipitate.